Journal: bioRxiv
Article Title: The multi-stage plasticity in the aggression circuit underlying the winner effect
doi: 10.1101/2024.08.19.608611
Figure Lengend Snippet: (A) Schematic of virus injection, light delivery and representative histology images. (B) Experimental timeline of slice recordings after LTP induction and the LTP protocol. (C) Experimental timeline of slice recordings after 1d or 10d winning paired with LTD induction and the LTD protocol. (D) Representative voltage-clamp recording traces of VMHvl Esr1 cells recorded from sham and 1d (D1), 5d (D2), and 10d (D3) LTP induction side. (E-F) The sEPSC frequency (E) and amplitude (F) of VMHvl Esr1 cells recorded from sham and 1d (E1, F1), 5d (E2, F2), and 10d (E3, F3) LTP induction side. (G) F-I curve of VMHvl Esr 1 cells recorded from sham and 1d (G1), 5d (G2), and 10d (G3) LTP induction side. (H) The maximal number of action potentials across current steps of VMHvl Esr1 cells recorded from sham and 1d (H1), 5d (H2), and 10d (H3) LTP induction side. (I) Representative voltage-clamp recording traces of VMHvl Esr1 cells recorded from sham and LTD induction side of mice with 1 day of winning experience. (J-K) The sEPSC frequency (J) and amplitude (K) of cells on the sham and LTD sides of mice with 1 day of winning experience. (L) F-I curve of cells recorded on the sham and LTD induction sides in mice with 10 days of winning experience. (M) Maximal number of action potentials of VMHvl Esr1 cells recorded on the sham and LTD sides in mice with 10 days of winning experience. (N) Illustration of different types of plasticity events that occurred over the course of repeated winning. (O) Virus injection and light delivery strategy for in vivo LTP induction in freely moving mice. (P) Histology images showing virus expression and optic fiber locations. (Q) Experimental timeline for testing aggressive behaviors using different types of intruders before and after 10d LTP induction. (R) The latency to attack BC male intruders before and after 10d LTP induction. (S) The duration of attacking BC intruders after 10 days of LTP induction vs. 10 days of winning. (T) The latency to attack DBA male intruders during the probing test after 10 days of LTP induction or 10 days of winning over BC intruders. (U) The number of C57 and SW mice that initiate the 1st attack during the competition test after 10d LTP induction or 10d winning over BC intruders. (V) The results of competition tests after 10 days of LTP induction or 10 days of winning over BC. (W) The latency to attack SW male intruders after 10 days of LTP induction or 10 days of winning over BC. Bar and error bar: mean ± SEM. Circles in E, F, H, J, K and M represent individual cells. Circles and lines in R, S, T, U, V, W represent individual animals. Numbers in parenthesis in E, F, G, H, J, K, L and M indicate the number of recorded cells. Numbers in parenthesis or inside of the bars in R-W indicate the number of subject animals. (E1, E2, F1, H1, H2, S) Unpaired t test. (E3, F2, F3, H3, J, K, M, T) Mann-Whitney test. (G1, G2, G3, L, R) Two-way ANOVA with repeated measures followed by Sidak’s multiple comparisons test. (U, V) Chi-square test. All statistical tests are two-tailed. All p :5 0.05 are specified. If not indicated, p > 0.05. See Supplementary Table 2 for detailed statistics. See also , , , and .
Article Snippet: Single-housed aggressive Swiss Webster male mice (>10 months, Taconic) were used as challengers to evaluate the maximum fighting ability of the test mice.
Techniques: Virus, Injection, In Vivo, Expressing, MANN-WHITNEY, Two Tailed Test
